Journal:

Article Title: An ImmunoChip prototype for simultaneous detection of antiepileptic drugs using an enhanced one-step homogeneous immunoassay 1 2 3 4

doi: 10.1016/j.ab.2007.03.019

Figure Lengend Snippet: ImmunoChip design and dispensing of the assay reagents. (A) A 3 × 3 multi-well ImmunoChip set-up and dimensions. (B) Dry ice was placed under the platform (XYZ stage), supporting a number of ImmunoChips, to achieve freezing of the assay reagents as soon as they were dispensed. In each well, the assay’s reagents were dispensed in the order (from the bottom to the top): (1) Beta-Glo solution with excipients (500 nl); (2) R2, containing ED-AED conjugate, and excipients (188 nl); and (3) R1, consisting of anti-AED antibody and EA, and excipients (250 nl). The reagents specific to the three AEDs were dispensed in individual rows (VPA, PHT, CBZ) in triplicate. ED, enzyme donor and EA, enzyme acceptor, are two genetically engineered fragments of β-galactosidase, essential for the CEDIA reaction. Ex, excipients present in the individual reagents are described in Materials and methods.

Article Snippet: The Beta-Glo assay system (Promega Corp., Madison, WI) consists of two parts that are combined at the time of assay to form Beta-Glo reagent, which contains biolumigenic substrate.

Techniques:

Journal:

Article Title: An ImmunoChip prototype for simultaneous detection of antiepileptic drugs using an enhanced one-step homogeneous immunoassay 1 2 3 4

doi: 10.1016/j.ab.2007.03.019

Figure Lengend Snippet: Determination of VPA, CBZ and PHT concentrations, in the presence of serum, by onestep CEDIA with Beta-Glo Assay System. In this set of experiments, drug-free normal serum was spiked with VPA, CBZ or PHT, as described in Materials and methods. Calibration curves represent slopes of kinetic curves (120~180 seconds) as a function of AED initial concentration. Crossed bars designate therapeutic ranges of the respective AED. Data are based on 4 replications for each concentration of respective drug measured (mean ± SD). CBZ (open diamond) and PHT (open square) employ lower X-axis; VPA (open circle) uses upper X-axis. RLU: Relative light unit(s).

Article Snippet: The Beta-Glo assay system (Promega Corp., Madison, WI) consists of two parts that are combined at the time of assay to form Beta-Glo reagent, which contains biolumigenic substrate.

Techniques: Glo Assay, Concentration Assay